Centro Andaluz de Biología Molecular y Medicina Regenerativa
Biological Resources Unit
The Biological Resources Unit enables animal experimentation in Cabimer providing the necessary resources under conditions required by national and EU legislation (Spanish RD 53/2013 and EU Directive 2010/63) for the protection of animals used for scientific purposes. The mission is to provide for the care, health and well-being of animals as well as to provide specialized techniques and equipment for research.
Cabimer has adhered to the Agreement on Transparency in Animal Experimentation promoted from Confederation of Scientific Societies of Spain (COSCE), with the collaboration of the European Association for Animal Research (EARA) and launched in 2016.

SCIENTIFIC COORDINATOR:
Dr. Luis Sánchez Palazón
TECHNICIENS:
Míriam González Fernández
Rosario Segarra Bermúdez
Gabriela Vázquez Salas
Joaquín González Magro
VETERINARIAN
Dra. Itziar Benito Latasa de Aranibar

Information

Researchers in Cabimer are using mouse models in a variety of ways, from basic research into disease mechanisms to translational research. The mission of the Biological Resource Unit is to provide for the care, health and well-being of animals as well as to provide specialized techniques and equipment for research.

The unit has capacity for some 6000 mice maintained in Specific Pathogen Free (SPF) condition, a health status monitored through a comprehensive health surveillance programme. Animal biosafety level 1 and 2 are available in our facility. Laboratory space and equipment is available for in vivo imaging, metabolic monitoring, inhalational anesthetic behavioural test, stereotaxic surgery, electroretinography, optical coherence tomography and transgenesis, including microinjection of DNA into zygotes and rederivation of transgenic lines by embryo transfer.

Services

Laboratory space and equipment can be booked through intranet. For other services, please contact with the unit

● Maintenance of mice in individual ventilated cage (IVC) racks of P1
● Maintenance of mice in individual ventilated cage (IVC) racks of P2
● Import and Export of mice
● Litter weaning, tissue biopsy, ear notching, detection of vaginal plugs
● Subcutaneous or intraperitoneal injection for administration of substances to mice
● Microinyection of DNA into the pronucleus of a fertilized egg and embryo transfer
● Consumable material: injectable anesthesia, hidrogel, boxes for the export of animals
● Rederivation of transgenic lines by embryo transfer
● In vitro fertilization
● Indirect calorimetry.
● In vivo imaging.
● Inhalation anesthesia for surgery.

● Coordination of the ethics committe for animal experimentation in Cabimer, a body authorized by the Government of Andalucía for the evaluation of research projects involving animal experimentation, according to RD 53/2013.

Equipment

● The heating, ventilation and air conditioning system of the unit controls ventilation, temperature and humidity within specified ranges in the animal rooms.
●Equipment in the washing area includes:autoclaves, rack washer and SAS with hydrogen peroxide conection.
● IVC racks
● In vivo imaging system Spectrum CT (PerkinElmer)
● OxyletPro system (Harvard Apparatus).
● Inhalation anesthesia system (Harvard Apparatus).
● Leica AM 6000 platform for micromanipulation.
● Newport anti-vibration table
● Microforge MF-900 (Narishige)
● Micropipette puller P-97 (Sutter Instruments)
● Microinjector CellTram Vario Eppendorf
● Microinjector CellTram Oil Eppendorf
● Primetech piezo impact drive PMM-150
● Femtojet microinjector Eppendorf
● Stereomicroscope Olympus with heating stage
● Cold light source (Schott)
● CO2 incubator
● Eppendorf centrifuge.

Fees

Service fees can be downloaded at this link or contact tf. +34 95 446 7438.