CABIMER has an extensive area dedicated to Cell Culture, distributed across three floors.
Tissue Culture Unit
SCIENTIFIC COORDINATOR:
TECHNICIEN COORDINATOR:
In the basement, the Unit has an area dedicated to non-human primary cell culture, adjacent to the Animal Facility, and a biosafety level P2 room for cell transduction.
En las restantes plantas, existen siete áreas destinadas a líneas celulares humanas y murinas y un área destinada al cultivo primario humano.
The Cell Culture Unit provides users with the ability to culture all types of animal cells, including both primary cultures and established cell lines, and equipment to facilitate their molecular and cellular studies.
The Culture Area is equipped with robust infrastructure to facilitate and streamline researchers’ work, including all the necessary equipment for cell culture, cell growth measurement, cell transfection, and cryopreservation.
The Cell Culture Unit currently:
- Facilitates users’ access to and utilization of the available facilities, ensuring the setup and maintenance of the infrastructure.
- Ensures compliance with work regulations to prevent culture contamination and promote good laboratory practices.
- Conducts regular inspections of the facilities and equipment.
- Monitors critical parameters in cell culture and manages cleaning, disinfection, and/or sterilization programs for the equipment used.
- Oversees the supply of CO₂ and nitrogen, both liquid and gaseous, throughout the center, and monitors the operation of cell cryopreservation tanks.
- Provides aliquots of heat-inactivated FBS, penicillin/streptomycin, glutamine, trypsin-EDTA, and sterile 1X PBS.
The scientific equipment of the Cell Culture Unit includes the following items:
Biosafety Cabinet
Funded by the R&D+i infrastructure and equipment grants for public entities, reference IE19-101-USE, awarded by the Regional Ministry of Economic Transformation, Industry, Knowledge, and Universities (PAIDI 2020).
Automated Fluorescence Cell Counter
Funded by the R&D+i infrastructure and equipment grants for public entities, reference IE19-101-USE, awarded by the Regional Ministry of Economic Transformation, Industry, Knowledge, and Universities (PAIDI 2020).
Liquid Nitrogen Cell Cryopreservation System
Funded by the R&D+i infrastructure and equipment grants for public entities, reference IE19-101-USE, awarded by the Regional Ministry of Economic Transformation, Industry, Knowledge, and Universities (PAIDI 2020).
Electroporation System for Cell Transfection in Culture
Funded by the R&D+i infrastructure and equipment grants for public entities, reference IE19-101-USE, awarded by the Regional Ministry of Economic Transformation, Industry, Knowledge, and Universities (PAIDI 2020).
Undercounter Freezer
Funded by the R&D+i infrastructure and equipment grants for public entities, reference IE19-101-USE, awarded by the Regional Ministry of Economic Transformation, Industry, Knowledge, and Universities (PAIDI 2020).
Others:
- Refrigerators and freezers
- Laminar flow cabinets
- Incubators
- Microscopes
- Automated cell counters
- Centrifuges
- Electroporators
- Water baths
- Shakers
Manuals and Guidelines
You can find the equipment user manuals and guidelines at this link.
Additionally, the center has two areas adjacent to the culture area dedicated to ultra-freezing for material cryopreservation, currently equipped with 6 liquid nitrogen containers with auto-injection systems.
You can consult the rates at this link or contact us by phone at 95 446 7744 or via email at mercedes.dana@cabimer.es.
