Centro Andaluz de Biología Molecular y Medicina Regenerativa
Cintia Roodveldt

e-mail: cintia.roodveldt@cabimer.es

‘Ramón y Cajal’ Investigator, University of Seville, Spain
CABIMER-Andalusian Center for Molecular Biology & Regenerative Medicine.

 

Information

Cintia Roodveldt, Ph.D.

‘Ramón y Cajal’ Investigator, University of Seville, Spain
CABIMER-Andalusian Center for Molecular Biology & Regenerative Medicine.
 

Misfolding proteins, molecular chaperones and their role in immune dysregulation linked to neurodegenerative disease

 

  • >2019: ‘Ramon y Cajal’ Investigator Programme. CABIMER. University of Seville.
  • >2009-2018: Assistant Professor, University of Seville. Department of Biochemistry, Molecular Biology & Immunology.
  • Visiting Scientist: Center for Neurologic Diseases (CND), Harvard Medical School, Boston, USA (2013, 2015); Princeton University, USA (2006); Laboratory of Molecular Biology (LMB), MRC, Cambridge, UK (2001, 2002), EMBO ST Fellowships.
  • 2011-2018: ‘Miguel Servet’ Investigator Programme. CABIMER, Seville. Neuroimmune Regulation Laboratory, Dept. of  Regeneration and Cell Therapy.
  • 2009-2010: Research Associate, CABIMER, Seville. JAE-Doc (CSIC) and ‘Juan de la Cierva’ Contract Award. University of Seville.
  • 2010: FEBS Distinguished Young Investigator Award.
  • 2007: Clare Hall Research Fellowship. Honorary.
  • 2006-2009: Postdoctoral Research Associate (PDRA), University of Cambridge, UK. Department of Chemistry (Lab: Prof. Chris M. Dobson, FRS). FEBS LT-Fellowship.
  • 2000-2005: PhD Student, The Weizmann Institute of Science, Rehovot, Israel. Department of Biological Chemistry (Lab: Prof. Dan S. Tawfik). Rodolfo May Fellowship Award.
  • 1999: B.Sc.,M.Sc. (Licenciatura) in Biotechnology, Universidad Nacional del Litoral, Santa Fe, Argentina. Fundación Antorchas Fellowship.

 

Research Grants (Principal Investigator)

  • 2019-2023: Spanish Ministry of Economy-MINECO.
  • 2019-2022: Fundación ‘Ramón Areces’, Spain.
  • 2015-2018: RETOS-Colaboración, Spanish Ministry of Economy-MINECO.
  • 2013-2015: Spanish Ministry of Economy-MINECO.

 

Major Research Interests

Protein misfolding diseases (PMDs), including Parkinson’s, Alzheimer’s, and Amyotrophic Lateral Sclerosis (ALS), are incurable and increasingly prevalent neurodegenerative disorders characterized by the aberrant misfolding, aggregation and deposition of specific proteins in certain areas of the brain, and ultimately leading to neuron death. The underlying pathogenic mechanisms of these highly complex pathologies are still poorly understood, but dysregulated immune responses involving exacerbated neuroinflammation and peripheral immune imbalance, are currently thought to play a major role in the development of disease.

In the last few years, a link has been established between a number of misfolding proteins, such as α-synuclein, β-amyloidand TDP-43, and exacerbated microglial activation and inflammation within the CNS, leading to neurodegeneration. The aim of  my research is to elucidate the molecular mechanisms involving misfolding proteins and heat-shock proteins —with prominent anti-aggregation functions and emerging immune-modulating activities— in immune dysregulation associated to neurodegenerative disease.

Address

Cintia Roodveldt
CABIMER-Andalusian Center for Molecular Biology & Regenerative Medicine
Av. Americo Vespucio 24, Parque Científico-Tecnológico Cartuja 93
41092 Seville, Spain

 Tel. +34 954467403

e-mail: cintia.roodveldt@cabimer.es

Links

PubMed
https:/www.ncbi.nlm.nih.gov/pubmed/?term=Roodveldt+C

ORCID
https:/orcid.org/0000-0003-4124-8769

Researcher ID
http:/www.researcherid.com/SID=E2xjJH4Wjo3eweU4uAO

Selected Publications

  •  Mannini B, Vecchi G, Labrador-Garrido A, Fabre B, Fani G, Franco JM, Lilley K, Pozo D, Vendruscolo M, Chiti F, Dobson CM, Roodveldt C. “Differential Interactome and Innate Immune Response Activation of Two Structurally Distinct Misfolded Protein Oligomers”. ACS Chem Neurosci. 10(8):3464-3478. 2019.

 

  •  Villadiego J, Labrador-Garrido A, Muñoz-Franco J, Leal-Lasarte MM, De Genst EJ, Dobson CM, Pozo D, Toledo-Aral JJ, Roodveldt C. “Immunization with α-synuclein/Grp94 reshapes peripheral immunity and suppresses microgliosis in a chronic Parkinsonism model”. Glia 66(1):191-205. 2018.

 

  • Leal-Lasarte MM, Franco JM, Labrador-Garrido A, Pozo D, Roodveldt C. “Extracellular TDP-43 aggregates target MAPK/MAK/MRK (MOK) kinase and trigger caspase-3/IL-18 signaling in microglia”. FASEB J.  31(7):2797-2816. 2017.

 

  • Sot B, Rubio-Muñoz A, Leal-Quintero A, Martinez-Sabando J, Marcilla M, Roodveldt C, Valpuesta JM. 2017. “The chaperonin CCT inhibits assembly of α-synuclein amyloid fibrils by a specific, conformation-dependent interaction”. Sci. Rep. 7:40859. 2017.

 

  • Labrador-Garrido A, Cejudo-Guillén M, Daturpalli S, Leal MM, Klippstein R, De Genst EJ, Dobson CM, Jackson SE, Pozo D, Roodveldt C. “Chaperome screening leads to identification of Grp94/Gp96 and FKBP4/52 as modulators of the α-synuclein-elicited immune response”. FASEB J. 30(2):564-77. 2016.

 

  • Caballero-Hernandez D, Toscano MG, Cejudo-Guillen M, Garcia-Martin ML, Lopez S, Franco JM, Quintana FJ, Roodveldt C, Pozo D. “The ‘Omics’ of Amyotrophic Lateral Sclerosis”. Trends Mol Med. 22(1):53-67. 2016.

 

  • Chen SW, Drakulic S, Deas E, Ouberai M, Aprile FA, Arranz R, Ness S, Roodveldt C, et al., Dobson CM, Cremades N. “Structural characterization of toxic oligomers that are kinetically trapped during α-synuclein fibril formation”. PNAS USA 112(16):E1994-2003. 2015.

 

  • Roodveldt C, Labrador A, Gonzalez-Rey E, Lachaud CC, Guilliams T et al., Dobson CM, Pozo D. “α-Synuclein preconditioning of microglia strongly affects the response induced by TLR stimulation”. PLoS One 8:e79160. 2013.

 

  • Mannini B, Cascella R, Zampagni M, van Waarde-Verhagen M, Meehan S, Roodveldt C, et al., Chiti F. “Molecular mechanisms used by chaperones to reduce the toxicity of aberrant protein oligomers”. PNAS USA 109:12479-84. 2012.

 

  • Roodveldt C, Bertoncini CW, Andersson A, van der Goot AT, Hsu ST, Fernandez-Montesinos R, de Jong J, van Ham TJ, Nollen EA, Pozo D, Christodoulou C, Dobson CM**. “Chaperone proteostasis in Parkinson’s disease: molecular insights into the Hsp70/α-synuclein complex”. EMBO J.  28, 3758-70. 12/1. 2009.

 

  • Roodveldt C & Tawfik DS. “Shared promiscuous activities and evolutionary features in various members of the amidohydrolase superfamily”. Biochem. 44, 12728-12736. 2005.

 

  • Aharoni A, Gaidukov L*, Khersonsky O, McQ Gould S*, Roodveldt C* & Tawfik DS. *Equally contributing authors. “The ‘evolvability’ of promiscuous protein functions”. Nat. Genet. 37, 73-76. 2005.